Abstract: Based on the method of integration operators, a new method of computing equivalent nodal forces and inner forces for prestressed non-uniform beams is presented in this paper. A non-uniform beam with multiple spans is treated as a single element, and the equivalent nodal forces and inner forces of multiple prestressed cables are directly computed. The proposed method successfully includes the influences of varied stiffness and shear deformation. It is an accurate method for computing prestressed non-uniform beam, which overcomes the shortage of previous methods.