Abstract: The quasi-zero stiffness isolation system has been widely studied because of its high static and low dynamic stiffness characteristics. However, the short quasi-zero stiffness range limits its application in the field of low-frequency vibration isolation. A new quasi-zero stiffness isolation system was designed based on the concept of parallel connection of nonlinear positive stiffness and negative stiffness, inspired by the Kresling origami pattern. The feasibility of the quasi-zero stiffness isolation system was analyzed and verified by the finite element method. The mechanical model of the origami-inspired quasi-zero stiffness system was established. The effects of the damping ratio, load amplitude and higher-order terms of the recovery force model on the amplitude-frequency characteristics and force transmissibility were studied under normal operating conditions. The vibration isolation performance analysis was also extended to overload operating conditions. The vibration isolation test of the quasi-zero stiffness system was carried out and compared with the finite element results. The analysis results show that the response amplitude and transmission rate of the vibration isolation system decrease as the damping ratio increases and the amplitude of the excitation force decreases. The recovery force model with higher-order terms enables the origami-inspired quasi-zero stiffness system to have a lower starting vibration isolation frequency, resonance frequency and vibration transmission rate under normal and overload conditions. The test results show that the origami-inspired quasi-zero stiffness system has good vibration isolation performance.