Abstract: In order to study the seismic performance of reinforced concrete shear walls in in-plane and out-of-plane directions under single main shock and different input directions of main-aftershock sequence, two shear wall specimens are designed for low cycle load tests to resist in-plane, in-plane-out-of-plane, out-plane and out-of-plane-in-plane forces respectively. The seismic performance of shear walls under different forces is studied by analyzing macroscopic failure phenomena and seismic performance parameters of specimens, and the damage status is evaluated. The specimens were simulated using finite element software, and two frame shear wall structural systems were designed for elasto-plastic time history analysis to study the deformation and damage of the shear wall in the two directions of the plane under single main shock and different input directions of the main-aftershock sequence. The results show that after damaged in in-plane direction, the shear wall’s seismic capacity decreases significantly when subjected to the force in out-of-plane direction, and the degree of damage caused by the excitation of the main-aftershock sequence is significantly higher than that caused by single main shock. Therefore, it is necessary to pay special attention to the seismic performance of shear walls in out-of-plane direction, and to study the seismic performance of the structural system under main-aftershock sequence with different input directions.