Abstract: To study the uniaxial stress fatigue performance of rubber concrete, equal cyclic load uniaxial stress fatigue tests were carried out. Rubber particles with a particle size of 30 meshes were used to replace sand by different dosages to prepare the rubber concrete. The Miner’s cumulative damage theory was used to define the damage quantity and establish the damage model of the rubber concrete fatigue strain. Probability and statistical methods are used to analyze the reliability of the test results of the fatigue life of rubber concrete. The distribution of the fatigue life of rubber concrete under uniaxial compressive constant amplitude cyclic loading is obtained. The results show that, with the same stress level, the fatigue life of rubber concrete is better than ordinary cement concrete and increases with the increase of rubber mixing quantity. The fatigue strain change of rubber concrete meets the three-stage law of ordinary concrete fatigue strain development. Its fatigue life follows the lognormal distribution. The linear regression analysis of the fatigue life of rubber concrete yields the P-S-N curve and fatigue limit strength.