Abstract: Through field and off-line tests, the vibration responses of the integral floating vibration isolation measures under the train excitation and fixed-point hammering excitation were tested, the differences of vibration isolation effects under the two kinds of load excitations were analyzed, and the effectiveness and accuracy of fixed-point hammering excitation was evaluated. The study results showed that: under the train and fixed-point hammering excitations, the vibration acceleration response of the floating isolation system could be significantly reduced. The characteristic peak value and energy distribution trend of the floating vibration isolation system under the two kinds of load excitations were similar. The vibration isolation quantity of weighted Z-Level under a hammering excitation was lower than that under a train excitation, and its vibration isolation effect was conservative. It could be seen that the actual train incentive mode is better than the fixed-point hammering incentive mode. A prediction method of vibration isolation effect for a flexible supported floating vibration isolation system was proposed, combined with the theoretical and experimental analysis, which can provide a reference for the evaluation and comparison of similar vibration isolation measures.