Abstract: A new type of coupler for connecting bars, non-slipping threaded sleeve assembly, is proposed, and based on this, replaceable energy dissipation connection is assembled. An axial low-cycle reversed loading test was carried out, indicating the reliability of load transfer and excellent energy dissipation performance. An innovative single-side yielding beam-column connection with replaceable energy dissipation bars (REDB-SYBC) is developed by placing the energy dissipation connection in the bottom of the beam end of the prefabricated concrete frame. The quasi-static test was carried out to evaluate the seismic behaviors of the connection, such as damage distribution, failure pattern, hysteretic characteristics, and energy dissipation capacity. The hysteretic curves of the specimens are stable and full without pinching, showing a good seismic performance. The experimental results show that: the single-side yielding deformation pattern of the REDB-SYBC connection minimizes the deformation of the adjacent floor slabs and gives full play to the advantage of energy dissipation capacity of connectors at beam bottom, and makes the main damage and failure occur on the energy dissipation bars. That realizes the design goal of damage concentration and the seismic design principle of “strong column and weak beam”. The energy dissipation bars can be replaced by the inner space of the non-slipping threaded sleeve assembly. The seismic behaviors of the repaired specimens are basically the same as those of the original specimens.