Abstract: This paper investigates the acceleration response spectrum approach for predicting dynamic responses of long-span structures due to individual running loading. The wireless in-insole was used to measure the individual running loads due to 372 records from 24 test subjects. Each record was applied to a single-degree-of-freedom system with various frequencies and damping ratios to determine its responses spectrum. The envelop curve of all responses spectra of records from the same subject was taken as his/her representative spectrum curve. Based on all the 24 representative curves, a design spectrum has been suggested, which comprises the first, second and third plateau and linear/exponential linking lines between each plateau. Spectrum parameters for different damping ratios and guarantee rates were determined based on the statistical analysis of experimental data. Furthermore, the effects of several factors on the spectrum were explored by numerical simulations and correction coefficients were accordingly developed, including floor span, multi-modes and boundary conditions. The exchange rules between different spectrum's predictions, e.g. peak value and root-mean-squares, were also established. Finally, a detailed application procedure of the spectrum approach was presented whose applicability and reliability have been validated by the comparisons with field measurements.