Abstract: In order to propose a shear deflection calculation method for trough girder with corrugated steel webs, the mechanical characteristics of composite girder under loads and the shearing stress distribution in sections are analyzed, a geometric equation of shear strain is deduced, and a computing method termed the “reaction forces-loads piecewise function” is proposed. A shear deflection calculation formula for trough girders with corrugated steel webs is established by integrating the geometric equation. This formula can be used to calculate the shear deflection of girders under several concentrated loads and uniform loads, and can be applied to corrugated steel web girders with many other section shapes. The shear deflection formula is verified through experimental tests and finite element analysis of four trough girders with corrugated steel webs and one corrugated steel web I-girder. Experimental results indicate that the calculated deflections of the five scaled beams are about 20% smaller than the test results without considering shear deformation, but the deflections calulated by the deflection formula considering shear deformation agree well with experimental results.