Abstract: According to the mechanical characteristics and damage characteristics of RC tandem roller isolation structures under earthquakes, the innovate connection joints suitable for the roller isolation structural column is proposed, and the seismic performance of the roller isolation structural column with innovate connection joints under earthquake of different intensities is studied by pseudo-dynamic test with scale ratio of 1/2. The uniform hazard design spectrum suitable for rolling isolation structures is formed, the roller isolation bearing is designed according to the structural displacement limit, and the damping effect of the roller isolation bearing is verified by numerical simulation. Ground motions are selected according to the uniform hazard design spectrum with different exceeding probabilities, pseudo-dynamic tests are carried out for the precast roller isolation structural column, the seismic performance indices including failure pattern, hysteretic curve, stiffness degradation and energy dissipation are analyzed under earthquakes of different intensities, and a cast-in-place isolation connection is also tested for the purpose of comparison. The results indicate that at the exceeding probability of 10%, the shear ratio of 0.5 is attained by numerical simulations of non-seismic and roller isolation bearing columns. The experimental results show a damage index of 0.279 of the precast roller isolation structural column at the exceeding probability of 2%, so the roller isolation bearing has a good damping effect under the excitation of rare earthquakes, and the damage to precast roller isolation bearing column is slight. At the exceeding probability of 2%, the maximum crack width develops to 1.8mm on cast-in-place column and 1.6mm on precast column. Comparing the skeleton curve, stiffness degradation and energy dissipation between precast column and cast-in-place column, it is shown that the bearing capacity, initial stiffness and cumulative hysteretic energy dissipation of precast column are slightly better than those of cast-in-place column.