Abstract: The change of penetration resistance of jacked piles in interactive soft and hard layered soil is analyzed. Different constitutive models have been adopted, including modified cam clay model for cohesive soil and Modified Drucker–Prager model for sand in order to simulate the penetration of jacked piles more reasonably in layered soil particularly in interactive cohesive and sandy layered soil with ABAQUS. Simultaneously, a series of other measures are taken to make the initial stress equilibrium in layered soil. Combining with an engineering case, the process of successive penetration of jacked pile in layered soil with significantly different properties and stiff soil-interlayer in soft soil is simulated and calculated successfully, with the variation cure of pile resistance with depth in penetration process being obtained, which can reflect the abrupt change phenomenon of stress around pile and jacking resistance. The conclusion of numerical analysis is consistent with the field observations. The research results can help probability analysis on penetration of jacked piles and pile squeezing effect.