Abstract: The statistic parameters of load effect and resistance to fatigue reliability of steel crane beams are studied in the paper. Based on statistic analysis of the measured data and other ovailable test data, the fatigue reliability of 8 configurations provided in the code, is calibrated by the first order second moment(FOSM). The target reliability beta and partial factor equation are proposed. An example is provided to verify the present method, which can be used in the evaluation of fatigue reliability and design of steel crane beams.