Abstract: A total of 97 high-rise reinforced concrete shear wall structures with the height between 45 m and 100m were selected for the vibration tests based on the environmental motivation method in order to identify the fundamental period, the second natural period and the third natural period in the horizontal and longitudinal directions. The regression analysis was employed to analyze the effect of the height-width ratio to the natural vibration period periods. The empirical formula for the natural vibration period and distribution range of the high-rise reinforced concrete shear wall were presented. The relation between a high order natural vibration period and the fundamental period were statistically developed. The formulas were verified by using two examples. The research is a supplement and perfection to the relevant regulations of the empirical formula of the structural natural vibration period in the Load code for the design of building structures (GB 50009—2012).