Abstract: The influence of the spatial variation of seismic ground motion on the seismic response of a continuous rigid frame bridge is studied. According to the EI-Centro wave adjusted with amplitude and a coherency model, the acceleration time histories of multiple supports inheriting the physical characteristics of the selected records are generated by utilizing the multivariate linear prediction (MLP) method, which falls into the conditional simulation algorithm. Then, acceleration time histories are exerted on the supports and the governing equations of motion of the bridge are solved by the large mass method. Finally, a long-span curved continuous rigid frame bridge with high piers subjected to earthquakes is taken as a case study in the consideration of the wave passage effect and full spatial variation. The numberical results show that the influence of full spatial variation should be considered in the seismic analysis of long-span bridges. In addition, the conditional simulation algorithm can be used in the seismic analysis of bridges.