An accurate material parameter of rubber has a key influence in tire FEA. Based on the image analysis, an entire test system was developed and applied to perform a uniaxial tension, biaxial tension and planar tension tests of rubbers usually used by truck tires. The stability, reliability and accuracy of some constitutive laws are researched by the basis of these test results, such as Polynomial, Ogden and Arruda-boyce etc. These results show that the biaxial tension and planar tension test are necessary for the inflation and static loading analysis of a TBR tire. The numerical simulation results prove that the contact pressure and the maximum strain energy density depend on the rubber constitutive parameters seriously and the Polynomial (2 order) and Ogden constitutive laws have better FEA results for tire rubber.