Abstract: In order to solve the problems of insufficient stiffness and strength of traditional horizontal members and low space utilization in multi-storey long-span structures, this study proposes a structural form of ultra-high performance concrete composite truss beams. Considering the nodal form of beam-column-frame rigid connections in a multi-storey large-span structure, the beam end often exists a large negative moment, and the steel-UHPC composite truss beam structure with solid web bearing is proposed to improve the force performance of the ends of the beam, so as to make it have a wider application value and significance. Based on the method of equivalent generation stiffness analysis, the calculation model and design methods of the composite truss beam are established through theoretical derivation. The research shows the results of the internal force distribution and magnitude, of the deflection, of the stress distribution are analyzed by ABAQUS modelling and compared with the theoretical ones. The analysis results show that: the results of finite element analysis and theoretical design methods are in a good agreement; it is also found that the steel-UHPC composite truss beam structure applied in multi-storey large-span structures shows better load carrying capacity and stiffness performance, and the bending stiffness of the combined joist beams is improved significantly; the provision of solid web bearings resulted in a significant reduction of peak stresses in the UHPC plate at the end-fixed bearings. The research results can provide a reference and a basis for the application and design analysis of steel-UHPC composite truss beams in multi-storey large-span structures.