Abstract: To study the stress characteristics and theoretical calculation formulas of the steel-concrete joint of railway hybrid girder, based on the existing calculation formulas of steel-concrete joint of highway bridges, the checking formulas for joint stress, shear connectors of top and bottom plates and load transmission of bearing plate of railway hybrid girders were put forward. Taking the Jialing River Bridge of Hanzhong to Bazhong & Nanchong Railway with a main span of 335 m as the research background, a finite element analysis (FEA) was conducted to explore the stress characteristics and the longitudinal and lateral stress distribution law of the joint, and then a comparative analysis was done between the formula and FEA. The FEA results show that the lateral stress distributions of steel and concrete of the joint at different cross-section are all uneven. The formula verification results indicate that the concrete of the joint is in compressive, meeting the requirements on section stress. The shear connector resistance coefficient ranges from 1.30 to 4.49, indicating adequate safety reserves. The resistance coefficient of bearing plates is between 3.18 and 10.08, showing that the resistance of front and rear bearing plates is much greater than the calculated load, and there is a room for optimization. The stresses of concrete and shear connectors on the top and bottom of the joint calculated by theoretical formula are generally similar to FEA results and can be used to calculate the anti-cracking/detachment and shear stress of steel-concrete joint after modification.