Abstract: In order to study the effect of longitudinal reinforcement ratio on the punching shear capacity of ordinary reinforced concrete slab-column connections, four slab-column interior connections with reinforcement ratios of 0.3%, 0.7%, 1.2% and 1.8% were made without stirrups, and punching shear tests were conducted. The test results show that with the increase of reinforcement ratio, the initial stiffness and ultimate capacity of slab-column connections increase significantly, while the ductility of specimens decreases. The damage mode changes from bending failure to punching failure gradually. The data of 269 punching shear tests on slab-column connections from both domestic and international sources were collected and organized, and then a comparative analysis was performed using codes from China, American, Europe, and Japan for punching shear capacity, which further emphasized the necessity of considering the longitudinal reinforcement ratio in the formulas. Through regression analysis of the experimental data and considering the major influencing factors, including the longitudinal reinforcement ratio, a calculation formula applicable to the punching shear capacity of reinforced non-stirrup concrete slab-columns was proposed.