Abstract: In order to study the vibration serviceability of large-span floor under the motivation of pedestrians,, the Gansu Provincial Gymnasium is studied as an example. The finite element model of this large-span suspension structure is established using ANSYS. The biodynamic model is simulated with stiffness-mass-damping (SMD) of single degree of freedom system, which is combined with the Fourier series load model to represent a single pedestrian load. Then the combined pedestrian load is loaded in the finite element model, and the acceleration response of the fixed-point loading, single-step moving loading and moving loading of population synchronization under the conditions of considering the pedestrian-structure coupling vibration is analyzed. The serviceability of the suspension structure is evaluated by the peak acceleration as the index. A single degree of freedom TMD and 3-DOF TMD are used to control the excessive vibration of the structure, and the damping effect of the single degree of freedom TMD and 3-DOF TMD is compared. The results show that 3-DOF TMD has good effect in controlling human induced vibration of large span floor structure.